Metallobetalactamase producing pseudomonas aeruginosa. Pdf first report of new delhi metallobetalactamase ndm1. Prevalence of extended spectrum betalactamase esbl and. Later this enzyme and its variants were detected in different gram negative bacilli in different countries. Multidrugresistant pseudomonas aeruginosa producing per1 extendedspectrum serine.
On november 1, 2016, a point prevalence survey was conducted at a chicago skilled nursing facility with ventilated residents to understand the prevalence of carbapenemase producing organisms in health care facilities in the chicago region. Respiratory infections associated with opportunistic bacteria such as metallobetalactamase producing p. Metallobetalactamase producer pseudomonas aeruginosa. Multidrugresistant pseudomonas aeruginosa producing per1. Endemic carbapenemresistant pseudomonas aeruginosa with acquired metallo.
Metallo beta lactamase production is an important mechanism for carbapenem resistance of pseudomonas aeruginosawhich, represents an emerging public health challenge. Dissemination of carbapenemresistance among multidrug. Its ability to acquire and combine different resistance determinants. A positive edtadisk synergy test suggested the production of a metallo lactamase. A prospective study was performed over a period of 8 months in a tertiary care hospital. The spread of genes enconding for these enzymes among gramnegative bacteria has been described in different countries as well as in different species and is a threat to public health. Class i integron, ericpcr, metallobeta lactamase, pseudomonas aeruginosa.
Premises including, equine production farm, bovine production. The study was conducted for the detection of beta lactamase genes which includes tem116, bla gim and bla bic in multi drug resistant pseudomonas aeruginosa from cancer patients. Metallo beta lactamases are responsible for carbapenemsresistant pseudomonas aeruginosa. Acinetobacter species from wound pus and sputum samples. Imipenemedta disk method for differentiation of metallo. A longitudinal survey from the uk did not show an increase in p. These genes play the most significant role in multi drug resistance. Prevalence of extendedspectrum beta lactamases, ampc beta lactamase and metallo beta lactamase producing pseudomonas aeruginosa and acinetobacter baumannii. In march 2012, a patient with history of prior hospitalisation in serbia was diagnosed in france with acute pyelonephritis due to ndm1 producing p. Metallobetalactamase mbl producing pseudomonas aeruginosa strains have been reported to be an important cause of nosocomial infections. Verona integronencoded metallobetalactamaseproducing. Coexistence of metallobetalactamaseencoding genes in.
In our study, the edtabased method was the only method able to detect all strains harboring the spm1 enzyme. A total of 5 042 protein sequences were concatenated applying neighbor joining, minimum evolution. Oct 23, 2017 extended spectrum beta lactamase and metalo beta lactamase production were observed in 24 55. Metallo beta lactamase, pseudomonas aeruginosa, multiresistance. Dominance of international highrisk clones among metallo. During 20002003, a total of 116 nonduplicate imipenemresistant p. Establishing clonal relationships between vim1like metallo. Metallo beta lactamase producing pseudomonas aeruginosa and acinetobacter baumannii. Multilocus sequence typing for metallo betalactamase producing p. This study aimed to characterize a comprehensive collection of mblproducing p. Extended spectrum and metalo betalactamase producing. Metallo beta lactamase mbl enzymes cause extensive drug. The survey identified 20 patients with verona integronencoded metallo beta lactamase producing carbapenemresistant pseudomonas aeruginosa.
Carbapenem resistance in pseudomonas aeruginosa is most commonly due to production of metallo beta lactamases mbls. In korea, approximately 10 and 50% of imipenem resistance in p. Epidemiology and characteristics of metallo lactamase. Metallobetalactamase mbl producing pseudomonas aeruginosa in the burn patients is a leading cause of morbidity and mortality and. A study on infections caused by metallo beta lactamase. Detection of esbl was done by the combined disk diffusion method as per clinical and laboratory. Detection of metallobetalactamase producing pseudomonas.
Prevalence of metallobetalactamaseproducing pseudomonas. There is not enough information regarding the prevalence of mblproducing p. Phenotypic detection of metallo and ampc lactamases. Health careassociated infections caused by metallo. The majority of patients were medical tourists who had weight loss surgery at a single facility in tijuana, mexico. Pcr experiments revealed the presence of the bla vim. Large cluster of verona integronencoded metallo beta lactamase producing carbapenemresistant pseudomonas aeruginosa isolates colonizing residents at a skilled nursing facility chicago, illinois, november 2016march 2018. Citrobacter freundii, pseudomonas aeruginosa, pseudomonas putida, acinetobacter spp. Pseudomonas aeruginosa pseudomonas aeruginosa metallo beta lactamase mbl positive salmonella enterica formerly known as salmonella choleraesuis serratia marcescens shigella dysenteriae staphylococcus aureus.
In this study, we used the disc diffusion method for the determination of antibiotic resistance against seven antibiotics and pcr technique for the. Nosocomial infections caused by multidrugresistant and carbapenemresistant pseudomonas putida isolates have been reported occasionally in severely ill or immunocompromised patients. In our present study, 74% of imipenem nonsensitive strains of acinetobacter baumannii were mbl producers. Increasing prevalence of imipenemresistant pseudomonas. Pseudomonas aeruginosa samples were widely resistant to most 36 antibiotics, but were sensitive for some antibiotics which may be recommended. Lagatolla c, tonin ea, montibragadin c, dolzani l, gombac f, bearzi c, et al. Here we report the microbiological characteristics of what are believed to be the two first carbapenemresistant vim metallo. Mbl producers were detected phenotypically by epsilometer test in both carbapenem susceptible and resistant p. Epidemiology and virulence of vim4 metallo beta lactamase producing pseudomonas aeruginosa isolated from burn patients in eastern algeria author links open overlay panel samah meradji a abouddihaj barguigua b c mohamed cherif bentakouk d kaotar nayme b c khalid zerouali c dekhil mazouz e houria chettibi a mohammed timinouni b. Pdf burn patients infected with metallobetalactamase. So, the main aims of this study were to determine the rates of biofilm production and metallo beta lactamase production mbl among the strains of pseudomonas aeruginosa and acinetobacter spp. Epidemiology and virulence of vim4 metallo beta lactamase producing pseudomonas aeruginosa isolated from burn patients in eastern algeria. Jun 30, 2015 metallobetalactamaseproducing gramnegative bacilli in korean nationwide surveillance of antimicrobial resistance group hospitals in 2003. Susceptibilities of clinical strains of imipenemintermediate or resistant pseudomonas aeruginosa isolated in the calgary health region from 2002 to 2004.
Clinical and microbiological cure was obtained under appropriate antibiotic treatment. This study was carried out to determine the prevalence of metallo. Clustering was performed by neighbourjoining method. As is typical with other mblproducing organisms, the isolate was shown to. The first reported mbl in korea was a vim2producing p. Delhi metallo beta lactamase 1 ndm1 producing pseudomonas aeruginosa. There is not enough information from india regarding their prevalence in diabetic foot ulcer dfu patients. In 2001, multidrugresistant pseudomonas aeruginosa carrying blavim mbl genes were found to be widespread approximately 20% of all p. Carbapenemresistant isolates of pseudomonas aeruginosa producing metallo. Metallo beta lactamase mbl producing pseudomonas aeruginosa strains have been reported to be an important cause of nosocomial infections. Class i integron, ericpcr, metallobetalactamase, pseudomonas aeruginosa. Those that possess metal at their active site are termed metallo. Metallobetalactamase mbl producing pseudomonas aeruginosa has emerged as a threat to hospital infection control, due to its multidrug.
Notably, it is a referred, highly indexed, online international journal with high impact factor. The aim of this study was to determine the prevalence and pattern of antibiotic resistance, extended spectrum and metallo beta lactamase producing p. A study of metallo beta lactamase producing clinical isolates of pseudomonas aeruginosa. Mar 06, 2011 this study was conducted to identify the. Treatment of infections caused by metallo b lactamase producing pseudomonas aeruginosa in the calgary health region m. Class 1 integron containing metallo beta lactamase gene bla imp1 in carbapenemresistant pseudomonas aeruginosa in thailand. Epidemiology and virulence of vim4 metallobetalactamase. In conclusion, it can be said that there is an upsurge of amp c beta lactamase producing pseudomonas aeruginosaand treatment should only be commenced only prior to knowing the sensitivity pattern of amp c isolates. Metallobetalactamase producing pseudomonas aeruginosa in.
Establishing clonal relationships between vim1like. Mbl can hydrolyze all metallo beta lactams except monobactams and are not inactivated by beta lactamase. Rapid communications recurrent pyelonephritis due to ndm1. Metallo beta lactamase an overview sciencedirect topics. Lactamase producing clinical isolates of acinetobacter species and pseudomonas aeruginosa from intensive care unit patients of a tertiary care hospital s irfan, a zafar, d guhar, t ahsan, r hasan abstract prompt detection of metallo. On the other hand, biofilm formation is one of the antibiotic resistance mechanisms in this bacterium. First identification of an escherichia coli clinical isolate. Hopkins 1, daniele meunier 1, jacqueline findlay 1, nazim mustafa 1, helena parsons 2, rachel pike 1, laura wright 1, neil woodford 1. Oxatype esbls have been found mainly in pseudomonas aeruginosa isolates from turkey and france.
Though there are several screening methods to detect this. Lactamaseproducing carbapenemresistant pseudomonas aeruginosa or vimcrpa. Pdf carbapenems are therapeutic choice against infections caused by. So, the main aims of this study were to determine the rates of biofilm production and metallo beta lactamase production mbl among the strains of pseudomonas aeruginosa. There is not enough information regarding the prevalence of mbl producing p. Biofilm and metallo betalactamase production among the.
Endemic carbapenemresistant pseudomonas aeruginosa with. In 1988, japan reported the first plasmidmediated mbl imp1 in pseudomonas aeruginosa 14. Detection of metallo beta lactamases among carbapenemresistant pseudomonas aeruginosa article pdf available in jundishapur journal of microbiology 78 september 2014 with 238 reads. Lactamase producing pseudomonas aeruginosa duck jin hong1, il kwon bae2, inho jang3, seok hoon jeong1, hyunkyung kang2, and kyungwon lee1 1department of laboratory medicine and research institute of bacterial resistance, yonsei university college of medicine, seoul.
Metallo beta lactamase mblmediated resistance to carbapenems is an emerging threat in hospital isolates of pseudomonas aeruginosa. The aim of this study was to investigate the molecular epidemiological characteristics of metallo. Lactamases mbls producing pseudomonas aeruginosa isolates. Pdf detection of metallobeta lactamases among carbapenem. Ten multidrugresistant pseudomonas aeruginosa strains producing vim1like acquired metallo. The strain was resistant to all lactams, including aztreonam, whereas the mic of imipenem and meropenem was 0. Docquier j, luzzaro f, amicosante g, toniolo a, rossolini g. Metallobetalactamase producing pseudomonas aeruginosa in a. During 20182019, cdc identified a multistate outbreak of a highly resistant strain of pseudomonas aeruginosa verona integronencoded metallo. Colistin is the only effective antibiotic in some cases of pseudo monas aeruginosa resistance to all tested antibiotics, even carbapenem.
Molecular analysis of metallo beta lactamase in multi drug. Pseudomonas aeruginosa samples were widely resistant to most 36 antibiotics, but were sensitive for some antibiotics which may be recommended 37 by physicians when treating pseudomonas aeruginosa. The aim of this study was to examine biofilm formation in colistinresistant p. Luzzaro f, mantengoli e, perilli m, lombardi g, orlandi v, orsatti a, dynamics of a nosocomial outbreak of multidrugresistant pseudomonas aeruginosa producing the per1 extendedspectrum beta lactamase. The spread of carbapenemresistant pseudomonas aeruginosa is a global concern. Clustering was performed by neighbour joining method. We report the case of a patient admitted to an intensive care unit, with sepsis caused by multidrugresistant sao paulo metallo beta lactamase 1 producing p. Detection of pseudomonas aeruginosa producing metallo. An increasing prevalence of carbapenem resistance mediated by acquired metallo. International journal of science and research ijsr is published as a monthly journal with 12 issues per year.